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Code
OSS00198W
$240USD
ID Tag
Rb1153-260909-WS
Immunogen
A synthetic peptide from n-terminal region of human 5HT5A receptor (Serotonin receptor 5A) conjugated to blue carrier protein was used as the antigen.
Accession
Also known
5-hydroxytryptamine receptor 5A, 5-HT5A, Serotonin receptor 5A, HTR5A
Target
FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.
Subcellular location: Cell membrane; Multi-pass membrane protein.
Subcellular location: Cell membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Rees S, et al. FEBS Lett. 355:242-246(1994)
Limitation
For research use only
Code
OSS00198W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for 5HT5A receptor.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised. This product contains 0.02% benzalkonium chloride.
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSS00198W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSS00198W
$240USD
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IHC on paraffin sections of human uterus. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
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IHC on paraffin sections of human brain tissue using Rabbit antibody to 5HT5A R: OSS00198W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain tissue using Rabbit antibody to 5HT5A R: OSS00198W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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