⚬ Services
Code
OSH00019W
$240USD    Buy Now 

ID Tag
Rb896-260409-WS

Immunogen
A synthetic peptide from the internal region of human HIF1 alpha conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 94% identity with rat and mouse sequences.

Accession

Also known
Hypoxia-inducible factor 1 alpha, HIF-1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, HIF1A, MOP1

Target
Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Subcellular location: Cytoplasm. Nucleus. Note: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
Tissue specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Wang G.L, et al. Proc. Natl. Acad. Sci. U.S.A. 92:5510-5514(1995)
2. Hogenesch J.B, et al. J. Biol. Chem. 272:8581-8593(1997)

Limitation
For research use only

Related
products
 
Code Product Name
OSH00020G Rabbit antibody to HIF1 alpha
 

Code
OSH00019W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for HIF1A.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSH00019W
$240USD    Buy Now 


Rabbit antibody to HIF1 alpha IHC on paraffin sections of rat kidney using Rabbit antibody to HIF1 alpha: OSH00019W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo's PT Module
Detection was done using Novolink HRP polymer from Leica following manufacturer's instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HIF1 alpha IHC on paraffin sections of human breast cancer tissue using Rabbit antibody to HIF1 alpha: OSH00019W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo's PT Module
Detection was done using Novolink HRP polymer from Leica following manufacturer's instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HIF1 alpha IHC on paraffin sections of human heart muscle tissue using Rabbit antibody to HIF1 alpha: OSH00019W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo's PT Module
Detection was done using Novolink HRP polymer from Leica following manufacturer's instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HIF1 alpha IHC on paraffin sections of human breast cancer tissue using Rabbit antibody to HIF1 alpha: OSH00019W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo's PT Module
Detection was done using Novolink HRP polymer from Leica following manufacturer's instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to HIF1 alpha
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuserâ„¢ OS2100
Red Carpet: A Histology Carpet for Slides