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Code
OSS00145W
$240USD    Buy Now 

ID Tag
Sh146-220309-WS

Immunogen
A synthetic peptide to phospho S633 of rat amiloride-sensitive sodium channel subunit beta (SCNN1B, SCNEB) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous with the corresponding sequence in mouse.

Accession

Also known
Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, Beta-NaCH, ENaCB, ENaCbeta

Target
FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2.
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells.
TISSUE SPECIFICITY: Lung and kidney.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Canessa C.M, et al. Nature 367:463-467(1994).
2. Kreutz R, et al. Hypertension 29:131-136(1997).
3. Henry P.C, et al. J. Biol. Chem. 278:20019-20028(2003).

Limitation
For research use only

Code
OSS00145W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
Sheep

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. Note that this antibody may also recognise the unphosphorylated form.

Specificity
Specific for SCNN1B.

Spcs X-react.
Rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Sheep antibody to phospho S633 SCNN1B
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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