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Code
OSN00021G
$240USD    Buy Now 

ID Tag
Sh129-070309-G

Immunogen
A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human, rat, zebrafish, drosophila and xenopus.

Accession

Also known
Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8

Target
Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein.
Subcellular location: Mainly nuclear
Tissue specificity: Ubiquitously expressed (at protein level).
Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Kumar S, et al. Biochem. Biophys. Res. Commun. 185:1155-1161(1992)
2. Kumar S, et al. Biochem. Biophys. Res. Commun. 195:393-399(1993)
3. Antenos M, et al. J. Biol. Chem. 277:44028-44034(2002)

Limitation
For research use only

Related
products
 
Code Product Name
OSN00020W Sheep antibody to NEDD8
 

Code
OSN00021G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
Sheep

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC (both on frozen and paraffin embedded tissues), WB. Use at a concentration of 10-50 µg/ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for NEDD8.

Spcs X-react.
Mouse, rat, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Sheep antibody to NEDD8
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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