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Code
OSN00018W
$240USD
ID Tag
Rb754-110109-WS
Immunogen
A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein has been used as the antigen. The peptide is homologous in human, rat, zebrafish, drosophila and xenopus.
Accession
Also known
Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8
Target
Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein.
Subcellular location: Mainly nuclear
Tissue specificity: Ubiquitously expressed (at protein level).
Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function
Subcellular location: Mainly nuclear
Tissue specificity: Ubiquitously expressed (at protein level).
Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Kumar S, et al. Biochem. Biophys. Res. Commun. 185:1155-1161(1992)
2. Kumar S, et al. Biochem. Biophys. Res. Commun. 195:393-399(1993)
3. Antenos M, et al. J. Biol. Chem. 277:44028-44034(2002)
2. Kumar S, et al. Biochem. Biophys. Res. Commun. 195:393-399(1993)
3. Antenos M, et al. J. Biol. Chem. 277:44028-44034(2002)
Limitation
For research use only
Code
OSN00018W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC (both on frozen and paraffin embedded tissues), WB. Use at a dilution of 1 : 300 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for NEDD8.
Spcs X-react.
Mouse, rat, human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSN00018W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Rabbit antibody to NEDD8
