A synthetic peptide from mouse MRGPRD conjugated to blue carrier protein was used as the antigen. The antigen shares 81% identity with rat's sequence.
Mas-related G-protein coupled receptor, member D, Beta-alanine receptor, G-protein coupled receptor TGR7, MRGD
FUNCTION: May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note: Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Dong X, et al. Cell 106:619-632(2001)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for MRGPRD.
Mouse. Other species not yet tested.
Lyophilised. This product contains 0.02% benzalkonium chloride.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.