⚬ Services
Code
OSS00103W
$240USD    Buy Now 

ID Tag
Rb716-221108-WS

Immunogen
A synthetic peptide from the 1t cytoplasmic domain of rat amiloride sensitive sodium channel subunit alpha (SCNN1A, SCNEA, Alpha-ENaC) conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
Epithelial a(+) channel subunit alpha, alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, alpha-NaCH, SCNN1, FLJ21883, ENaCalpha, Alpha-ENaC, Alpha ENaC

Target
FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells.
TISSUE SPECIFICITY: Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart.
INDUCTION: By aldosterone.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Voilley N, et al. Proc. Natl. Acad. Sci. U.S.A. 91:247-251(1994).
2. Snyder P.M, et al. J. Biol. Chem. 277:5-8(2002).

Limitation
For research use only

Code
OSS00103W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SCNN1A.

Spcs X-react.
Rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to SCNN1A
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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