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Code
OSG00033G
$240USD
ID Tag
Rb659-060908-G
Immunogen
A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.
Accession
Also known
38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase
Target
FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.
CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Fort P, et al. Nucleic Acids Res. 13:1431-1442(1985).
Limitation
For research use only
Code
OSG00033G
$240USD
Unit size
500 µg
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB. A concentration of 1-5 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for GAPDH.
Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSG00033G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSG00033G
$240USD
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WB on marmoset tissue lysates. Lysates were prepared in Ripa buffer containing 1% Triton X-100, 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:20000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
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WB on rat and mouse tissue lysates. Lysates were prepared in Ripa buffer containing 1% Triton X-100, 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:30000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
⚬ Products
Rabbit antibody to GAPDH
