⚬ Services
Code
OSP00021W
$240USD    Buy Now 

ID Tag
Rb466-150608-WS

Immunogen
A synthetic peptide from internal region of human Piccolo (Aczonin) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
Aczonin

Target
FUNCTION: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
SUBUNIT: Interacts with Rabac1/Pra1, RIMS2 and profilin.
SUBCELLULAR LOCATION: Cell junction, synapse. Note: Concentrated at the presynaptic side of synaptic junctions.
TISSUE SPECIFICITY: Highly expressed in brain. Moderately expresssed in pituitary gland and pancreatic islets. Low levels found in stomach.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Wang X, et al. J. Cell Biol. 147:151-162(1999).

Limitation
For research use only

Related
products

Code
OSP00021W
$240USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Piccolo.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSP00021W
$240USD    Buy Now 


Rabbit antibody to Piccolo IF on 1 day-old mouse cortical cell culture using Rabbit antibody to Piccolo (Aczonin) (OSP00021W) at 1: 2000 dilution. DAPI counter stained.
Rabbit antibody to Piccolo IF on 1 day-old mouse cortical cell culture using Rabbit antibody to internal part of Piccolo (Aczonin): whole serum (OSP00021W) at 1: 2000 dilution. DAPI counter stained appearing in blue.
Rabbit antibody to Piccolo WB on mouse brain lysate. Blocking with 0.5% LFDM for 30 min at RT; Primary antibody used at 1:1000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the detail
⚬ Products
Rabbit antibody to Piccolo
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

Watch Videos

Autoperfuserâ„¢ OS2100
Red Carpet: A Histology Carpet for Slides