⚬ Services
Code
OSC00060W
$240USD    Buy Now 

ID Tag
Rb688-060908-WS

Immunogen
A synthetic peptide from the n-terminal of human VPS24 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
Chromatin-modifying protein 3, Vacuolar protein-sorting-associated protein 24, hVps24, Neuroendocrine differentiation factor, Charged multivesicular body protein 3, VPS24, NEDF

Target
FUNCTION: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. Required for sorting/trafficking of EGF receptor. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins.
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Membrane; Lipid-anchor.
TISSUE SPECIFICITY: Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Wilson E.M, et al. J. Clin. Endocrinol. Metab. 86:4504-4511(2001).
2. Hillier L.W, et al. Nature 434:724-731(2005).

Limitation
For research use only

Related
products
 
Code Product Name
OSC00122G Rabbit antibody to VPS24
 

Code
OSC00060W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specificity
Specific for CHMP3.

Spcs X-react.
Mouse, rat, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSC00060W
$240USD    Buy Now 


Rabbit antibody to VPS24 WB on rat brain lysate using Rabbit antibody to VPS24 (OSC00060W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

⚬ Products
Rabbit antibody to VPS24
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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