⚬ Services
Code
OSN00011W
$240USD    Buy Now 

ID Tag
Rb710-060908-WS

Immunogen
A synthetic peptide from the HECT domain of human NEDD4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Neural precursor cell expressed developmentally down-regulated protein 4, E3 ubiquitin-protein ligase NEDD4, NEDD-4

Target
FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
FUNCTION: Involved in the embryonic development and differentiation of the central nervous system.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Hatakeyama S, et al. J. Biol. Chem. 272:15085-15092(1997).

Limitation
For research use only

Code
OSN00011W
$240USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for NEDD4.

Spcs X-react.
Mouse, rat, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to HECT domain of NEDD4
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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