⚬ Services
Code
OSN00009W
$240USD    Buy Now 

ID Tag
Rb687-060908-WS

Immunogen
A synthetic peptide from the n-terminal (cytoplasmic) domain of mouse NEDD4 WW domain binding protein 5 (Ndfip1, NFIP1) conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 96% identity with human and rat Ndfip1.

Accession

Also known
NEDD4 family-interacting protein 1, Ndfip1, N4wbp5, NFIP1

Target
FUNCTION: May play a role in Golgi structure maintenance.
SUBUNIT: Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Jolliffe C.N, et al. Biochem. J. 351:557-565(2000).
2. Carninci P, et al. Science 309:1559-1563(2005).
3. Harvey K.F, et al. J. Biol. Chem. 277:9307-9317(2002).

Limitation
For research use only

Code
OSN00009W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 500 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Ndfip1.

Spcs X-react.
Mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to NDFIP1
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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