⚬ Services
Code
OST00223G
$260USD    Buy Now 

ID Tag
Sh100-060908-G

Immunogen
A synthetic peptide from aa region 730-821 (including phospho Y816 site) of rat TrkB conjugated to ablue carrier protein was used as the antigen. The peptide is homologous in human and mouse.

Accession

Also known
Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB/GP95-TrkB, Trk-B, BDNF/NT-3 growth factors receptor

Target
FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and ARMS.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Klein R, et al. EMBO J. 8:3701-3709(1989).

Limitation
For research use only

Related
products

Code
OST00223G
$260USD    Buy Now 

Unit size
500 ug

Conjugate
Unconjugated antibody

Host
Sheep

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user. This antibody is also likely to recognise the unphosphorylated form.

Specificity
Specific for TrkB.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Sheep antibody to phospho Y816 TrkB
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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