A synthetic peptide from internal region of human Histone deacetylase 1 (HDAC1, RPD3L1) conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and moue.
FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
CATALYTIC ACTIVITY: Hydrolysis of an N6-acetyl-lysine residue of a histone to yield a deacetylated histone.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Taunton J, et al. Science 272:408-411(1996).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 500 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specific for HDAC1.
Human, Mouse, rat. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.