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Code
OST00217W
$240USD    Buy Now 

ID Tag
Sh114-140908-WS

Immunogen
A synthetic from the 4th cytoplasmic loop of human transient receptor potential cation channel subfamily A member 1 (TRPA1, ANKTM1) conjugated to an immunogenic carrier protein was used as the antigen.
The location of the immunogen is highlighted in green.

Accession

Also known
Transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains protein 1, transformation sensitive protein p120, ANKTM1

Target
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
FUNCTION: Receptor-activated non-selective cation channel involved in detection of sensations such as cold, pain or sounds perception. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Also involved in pain sensation triggered by capsaicin, the pungent ingredient in chilli peppers. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
TISSUE SPECIFICITY: Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
MISCELLANEOUS: Inhibited by ruthenium red, a potent blocker of TRPV channels.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Jaquemar D, et al. J. Biol. Chem. 274:7325-7333(1999).
2. Dai Y, et al. J. Clin. Invest. 117 (7), 1979-1987 (2007).
3. Hinman A, et al. Proc. Natl. Acad. Sci. U.S.A. 103 (51), 19564-19568 (2006).
4. Clapham D.E, et al. Pharmacol. Rev. 57 (4), 427-450 (2005).
5. Jaquemar D, et al. J. Biol. Chem. 274 (11), 7325-7333 (1999).

Limitation
For research use only

Related
products

Code
OST00217W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
Sheep

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Specificity
Specific for TRPA1.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Code
GST00020P
$120USD   

ID Tag
GS 50278-50

Unit size
50 µg

Accession

Also known
Transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains protein 1, transformation sensitive protein p120, ANKTM1

Target
This is the immunising peptide for the products Rabbit antibody to TRPA1 (OSR00130W), Rabbit antibody to TRPA1 (OST00060G)

Purity
>88%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is mostly soluble in basic buffers (pH 8-9).

Storage
Maintain refrigerated at 2-8°C for up to 12 months. After reconstitution keep at -20°C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSR00130W,OST00060G

⚬ Products
Sheep antibody to TRPA1
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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