A synthetic peptide from aa region 790-839 of the 4th cytoplasmic domain of rat TRPV1 conjugated to blue carrier protein was used as the antigen.
The location of the epitope is highlighted in
Transient receptor potential cation channel subfamily V member 1,
TrpV1, osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid receptor type 1-like, Capsaicin receptor, transient receptor potential cation channel, subfamily V, member 1, Vr1, Vr1l1, VR.5'sv
FUNCTION: Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid, and endocannabinoids, like anandamide, and bradykinin.
SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in trigeminal and dorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain and peripheral blood mononuclear cells.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Garcia-Martinez C, et al. J. Biol. Chem. 275:32552-32558(2000).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 500 is recommended. The optimal dilution should be determined by the end user.
Specific for TRPV1.
Rat, mouse. Other species not yet tested.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.