⚬ Services
Code
OSS00079W
$240USD    Buy Now 

ID Tag
Rb569-100808-WS

Immunogen
A synthetic peptide from aa region 390-440 of the extracellular domain of human SCNN1A conjugated to blue carrier protein was used as the antigen.

Accession

Also known
Epithelial a(+) channel subunit alpha, alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, alpha-NaCH, SCNN1, FLJ21883, ENaCalpha, Alpha-ENaC

Target
FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells.
TISSUE SPECIFICITY: Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart.
INDUCTION: By aldosterone.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Voilley N, et al. Proc. Natl. Acad. Sci. U.S.A. 91:247-251(1994).
2. McDonald F.J, et al. Am. J. Physiol. 266:L728-L734(1994).

Limitation
For research use only

Code
OSS00079W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SCNN1A.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Code
OSS00079P
$120USD   

ID Tag
53191-19

Unit size
50 µg

Accession

Also known
Epithelial a(+) channel subunit alpha, alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, alpha-NaCH, SCNN1, ENaCalpha, Alpha-ENaC

Target
This is the immunising peptide for the product Rabbit antibody to SCNN1A (OSS00079W).

Purity
>85%

Applications
Control peptide for the product OSS00079W.

Format
Lyophilised

Reconstitution
Dissolve in DMSO prior to addition of PBS to achieve the desired concentration.

Storage
Maintain refrigerated at 2-8°C for up to 12 months. After reconstitution keep at -20°C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSS00079W

⚬ Products
Rabbit antibody to SCNN1A (390-440)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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