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Code
OSS00109W
$240USD    Buy Now 

ID Tag
Rb2776-080616-WS

Immunogen
A synthetic peptide from mouse SUMO 1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in human, rat and marmoset.

Accession

Also known
Small ubiquitin-related modifier 1, SUMO-1, Sumo1

Target
Function: Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4
Subcellular location: Nucleus. Cytoplasm
Post-translational modification: Cleavage of precursor form by SENP1 or SENP2 is necessary for function.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Um J.W, et al. J. Neurosci. Res. 84:1543-1554(2006)

Limitation
For research use only

Related
products
 
Code Product Name
OSS00110G Rabbit antibody to SUMO1
 

Code
OSS00109W
$240USD    Buy Now 

Unit size
150 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 500 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SUMO1.

Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 150 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00109W
$240USD    Buy Now 


Rabbit antibody to SUMO1 WB on rat brain lysate. Lysate was prepared in Ripa containing 1% Triton X-100, 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to SUMO1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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