Osenses Protocols

 

 

 

5x SDS Loading Buffer

Component

Add

Final Amount

Tris (2 M), pH 6.8

12.5 ml

250 mM

MQ water 

17 ml

 

Glycerol

50 ml

50%

SDS

10 g

10%

2-ME

12.5 ml

12.5%

Pink-Q colour (Osenses)

Add to the lysate after heat denaturing.

 

Total volume

(add MQ water up to) 100 ml

Add Tris, water and glycerol while stiring and heat upp the solution to 40-50C then slowly add the SDS and continue stirring (stop the heating), add the 2-ME and stir until the solution becomes translucent, it might take 1-2 hours. Apply heat/stir the solution until clear. Finally, adjust the volume with MQ water to 100 ml.

Keep at Room Temperature for short term and for longer term keep at -20C.

Pink-Q colour can be added after the sample has been heat-denatured. Add 1 ul per 100 ul of sample.