⚬ Services
Code
OSB00020W
$240USD    Buy Now 

ID Tag
Rb3148-241117-WS

Immunogen
A synthetic peptide from human Beclin1 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Beclin-1, BECN1, GT197, ATG6, VPS30, beclin1

Target
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in autophagy.
SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells
TISSUE SPECIFICITY: Ubiquitous

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Feng W, et al. J. Mol. Biol. 372 (1), 223-235(2007).

Limitation
For research use only

Code
OSB00020W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a dilution of 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Beclin-1.

Spcs X-react.
Human, mouse, rat, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSB00020W
$240USD    Buy Now 


Rabbit antibody to Beclin1 WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.
Rabbit antibody to Beclin1 WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.
Rabbit antibody to Beclin1 WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.
Rabbit antibody to Beclin1 WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to Beclin1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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