⚬ Services
Code
OSA00261W
$240USD    Buy Now 

ID Tag
Rb2260-271213-WS

Immunogen
A synthetic peptide from aa region 200-250 of human Aggrecan conjugated to blue carrier protein was used as the antigen. The peptide is homologous with rat and mouse sequences.

Accession

Also known
Cartilage specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2

Target
FUNCTION: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
SUBCELLULAR LOCATION: Secreted extracellular space extracellular matrix
Tissue specificity: Restricted to cartilages

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Morawski M1, et al. Int J Biochem Cell Biol. 2012 May;44(5):690-3

Limitation
For research use only

Related
products

Code
OSA00261W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Aggrecan.

Spcs X-react.
Human. Other species not yet tested but expected to work in rat and mouse.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00261W
$240USD    Buy Now 


Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Aggrecan (200-250) IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to Aggrecan (200-250)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser � OS2100
Red Carpet: A Histology Carpet for Slides