A synthetic peptide from human SLC5A8 conjugated to blue carrier protein was used as the antigen.
Electrogenic sodium monocarboxylate cotransporter, Solute carrier family 5 member 8, SMCT1, SMCT, SLC5A8
FUNCTION: Acts as an electrogenic sodium (Na+) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na+:substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na+ gradient and hence contribute to the maintenance of the energy status and function of neurons.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Note: Restricted to the apical cell membrane of enterocytes.
Tissue specificity: Expressed in brain, colon, kidney and in the ileum and jejunum of small intestine. In the kidney, expression occurred in the proximal tubule and the loop of Henle, being restricted to tubular epithelial cells in both the cortex and the medulla. In the colon, predominantly expressed in the distal half of the large bowel and in the most terminal ileum. Localized selectively in the luminal surface of crypts in the large intestine and to the brush border in the middle parts of crypts in the cecum. In the brain, expression was seen throughout, exclusively in neurons, including the cortex, hippocampus, cerebellum and pituitary gland (at protein level). Expression is reduced in oligodendrogliomas.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Rodriguez A.-M, et al. J. Clin. Endocrinol. Metab. 87:3500-3503(2002)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 1000 for IHC-P and 1:2000 for WB is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for SLC5A8.
Human, marmoset. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.