A synthetic peptide from aa region 150-200 of human Bif conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
SHLB1, SH3 domain-containing GRB2-like protein B1, Bax-interacting factor 1, Bif-1, SH3GLB1, Bif, Bif1, Bif 1
Function: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.
Subcellular location: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note: Association with the Golgi apparatus depends on the cell type
Tissue specificity: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Cuddeback S.M, et al. J. Biol. Chem. 276:20559-20565(2001)
2. Modregger J, et al. J. Biol. Chem. 278:4160-4167(2003)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 8000 is recommended fro WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for Bif.
Human, rat, mouse. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.