⚬ Services
Code
OSG00033G
$240USD    Buy Now 

ID Tag
Rb659-060908-G

Immunogen
A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

Accession

Also known
38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase

Target
FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Fort P, et al. Nucleic Acids Res. 13:1431-1442(1985).

Limitation
For research use only

Code
OSG00033G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 1-5 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for GAPDH.

Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSG00033G
$240USD    Buy Now 


Rabbit antibody to GAPDH WB on marmoset tissue lysates. Lysates were prepared in Ripa buffer containing 1% Triton X-100, 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:20000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to GAPDH WB on rat and mouse tissue lysates. Lysates were prepared in Ripa buffer containing 1% Triton X-100, 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:30000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to GAPDH
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides