⚬ Services
Code
OSA00056W
$240USD    Buy Now 

ID Tag
Rb1441-120610-WS

Immunogen
A synthetic peptide from mouse ATP1A1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Accession

Also known
AT1A1, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Atp1a1

Target
FUNCTION: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Melanosome.
CATALYTIC ACTIVITY: ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).
SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Vosseller K, et al. Proteomics 5:388-398(2005).

Limitation
For research use only

Code
OSA00056W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 25000 is recommended for WB and 1:10000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. Recommended tissue lysing buffer: Ripa, containing 1% SDS but no SDC.

Specificity
Specific for Na+/K+ -ATPase alpha 1 subunit.

Spcs X-react.
Mouse, rat, human, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00056W
$240USD    Buy Now 


Rabbit antibody to ATP1A1 WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:25000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to ATP1A1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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