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Code
OSH00004W
$240USD    Buy Now 

ID Tag
Rb2601-230615-WS

Immunogen
A synthetic peptide from the internal region of rat HOMER3 conjugated to blue carrier protein was used as the antigen.

Accession

Also known
Home3, Homer protein homolog 3, VASP/Ena-related gene up-regulated during seizure and LTP 3, Vesl-3, Vesl3

Target
FUNCTION: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors it aids the coupling of surface receptors to intracellular calcium release.
SUBUNIT: Encodes coiled-coil structures that mediate homo- and heteromultimerization.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note: Postsynaptic density of neuronal cells.
It may interact with group I metabotropic glutamate receptors and regulate their coupling to membrane ion channels.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Kammermeier, P. J, et al. J. Neurosci. 20 (19), 7238-7245(2000).

Limitation
For research use only

Code
OSH00004W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 2000 is recommended fro WB and 1:1000 for IHC-P. The optimal dilution should be determined by the end user.

Specificity
Specific for Homer3.

Spcs X-react.
Rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSH00004W
$240USD    Buy Now 


Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 WB on rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to HOMER3 IHC-P on paraffin sections of rat hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
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Rabbit antibody to HOMER3
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Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
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