A synthetic peptide from extracellular domain of human EAAT3 (aa region 150-200) conjugated to an immunogenic carrier protein was used as the antigen. The peptide shares 96% identity with rat and mouse sequence.
Sodium-dependent glutamate/aspartate transporter 3, Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Solute carrier family 1 member 1, EAAC1, EAAT3, SLC1A1
FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Defects in SLC1A1 may be a cause of dicarboxylicamino aciduria; also known as glutamate-aspartate transport defect. This is as defect in renal and probably intestinal transport of glutamic and aspartic acids and is associated with moderate hyperprolinemia.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Shashidharan P, et al. Brain Res. 662:245-250(1994)
For research use only
NZ white rabbit
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Specific for EAAT3.
Human, rat, mouse. Other species not yet tested.
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.