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Code
OST00091W
$240USD    Buy Now 

ID Tag
Rb766-140209-WS

Immunogen
A synthetic peptide from the 1t cytoplasmic loop of human TRPV1 conjugated to blue carrier protein was used as the antigen.
The location of the epitope is highlighted in green.

Accession

Also known
TrpV1, osm-9-like TRP channel 1, OTRPC1, vanilloid receptor 1, capsaicin receptor, VR1, transient receptor potential cation channel subfamily V member 1

Target
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.
FUNCTION: Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL.
Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Hayes P, et al. Pain 88:205-215(2000).

Limitation
For research use only

Code
OST00091W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for TRPV1.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OST00091W
$240USD    Buy Now 


Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRPV1 IHC-P on paraffin sections of human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
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Rabbit antibody to TRPV1
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Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
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