A synthetic peptide from rat SNAP-25 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and human.
SNAP-25, Synaptosomal-associated 25 kDa protein, SNAP25, RIC4, SEC9, SNAP, RIC-4, Super protein, SUP
FUNCTION: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1, SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation.
TISSUE SPECIFICITY: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Madison D.L, et al. J. Neurochem. 72:988-998(1999).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for SNAP-25.
Rat, mouse, human. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.