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Code
OSS00021W
$240USD    Buy Now 

ID Tag
Rb311-200308-WS

Immunogen
A synthetic peptide from rat Synaptophysin conjugated to blue carrier protein was used as the antigen.

Accession

Also known
SYP

Target
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.
FUNCTION: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Leube R.E, et al. EMBO J. 6:3261-3268(1987).

Limitation
For research use only

Related
products

Code
OSS00021W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1000 is recommended for WB and 1:3000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Synaptophysin.

Spcs X-react.
Rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00021W
$240USD    Buy Now 


Rabbit antibody to Synaptophysin IHC-P on paraffin sections of rat brain.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Synaptophysin WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:10000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to Synaptophysin IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
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Rabbit antibody to Synaptophysin
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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