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Code
OSS00018W
$240USD    Buy Now 

ID Tag
Rb308-200308-WS

Immunogen
A synthetic peptide from aa region 440-490 of mouse Synapsin III conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

Accession

Also known
Synapsin 3, Syn3, Synapsin III

Target
FUNCTION: May be involved in the regulation of neurotransmitter release and synaptogenesis. Binds ATP with high affinity and ADP with a lower affinity. This is consistent with a catalytic role of the C-domain in which ADP would be dissociated by cellular ATP after bound ATP was hydrolyzed.
SUBUNIT: Interacts with CAPON.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Peripheral membrane protein localized to the cytoplasmic surface of synaptic vesicles.
MISCELLANEOUS: Regulated by calcium. Calcium inhibits ATP binding to the C-domain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Ehringer M.A, et al. Mamm. Genome 12:657-663(2001).
2. Trinidad J.C, et al. Mol. Cell. Proteomics 5:914-922(2006).

Limitation
For research use only

Code
OSS00018W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Specificity
Specific for Synapsin-3.

Spcs X-react.
Rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00018W
$240USD    Buy Now 


Rabbit antibody to Synapsin 3 (440-490) IHC on rat sciatic nerve using Rabbit antibody to Synapsin III (OSS00018W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

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Rabbit antibody to Synapsin 3 (440-490)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
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