⚬ Services
Code
OSA00027W
$240USD    Buy Now 

ID Tag
Rb264-270108-WS

Immunogen
A synthetic peptide from aa region 20-70 of drosophila ATG12 conjugated to blue carrier protein has been used as the antigen.

Accession

Also known
APG12-like, HAPG12, APG12, autophagy related 12 homolog

Target
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. Following conjugation to ATG5, the conjugate participates in the formation of autophagosome.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Adams M.D, et al. Science 287:2185-2195(2000).
2.Scott R.C, et al. Dev. Cell 7:167-178(2004).

Limitation
For research use only

Code
OSA00027W
$240USD    Buy Now 

Unit size
150 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a dilution of 1:300 to 1: 2000. The optimal dilution should be determined by the end user.

Specificity
Specific for ATG12.

Spcs X-react.
Drosophila, other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to ATG12 (20-70)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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