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Code
OST00025W
$240USD    Buy Now 

ID Tag
Sh33-060108-WS

Immunogen
A synthetic peptide from rat TRPC4 (isoforms: alpha, beta, beta-2, theta, iota, kappa) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse, human and many other species.
The location of the epitope is highlighted in green.

Accession

Also known
Short transient receptor potential channel 4, TrpC4, Trp4, Capacitative calcium entry channel 1, CCE1, Trrp4, Receptor-activated cation channel TRP4, Trp-related protein 4, hTrp-4, hTrp4

Target
FUNCTION: Thought to form non-selective a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion.
SUBUNIT: Isoform alpha but not isoform beta associates with inositol-1,4,5-triphosphate receptor (ITPR). Interacts with NHERF.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
- TISSUE SPECIFICITY: Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb). Lower levels are detected in other tissues.
- TISSUE SPECIFICITY: Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Funayama M, et al. Brain Res. Mol. Brain Res. 43:259-266(1996).

Limitation
For research use only

Code
OST00025W
$240USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
Sheep

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Specificity
Specific for TRPC4. See also the immunogen.

Spcs X-react.
Rat, mouse, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Code
GST00015P
$0USD   

ID Tag

⚬ Products
Sheep antibody to TRPC4
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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