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Code
DOM00001G
$240USD    Buy Now 

ID Tag
Rb2141-040913-G

Immunogen
Native myeloperoxidase isolated from human leucocytes

Accession

Also known
Myeloperoxidase

Target
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
SUBCELLULAR LOCATION: Lysosome

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Morishita K, et al. J. Biol. Chem. 262:3844-3851(1987).

Limitation
For research use only

Related
products

Code
DOM00001G
$240USD    Buy Now 

Unit size
500 ug

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Protein G purified IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A working concentration of 10-50 ug/ml is recommended. The optimal dilution should be determined by the end user. This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins.

Specificity
Specific for MPO.

Spcs X-react.
Human

Format
Lyophilised

Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
DOM00001G
$240USD    Buy Now 


Rabbit antibody to MPO Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and  followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue).  Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X
Rabbit antibody to MPO Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X
Rabbit antibody to MPO 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).
Rabbit antibody to MPO Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X
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Rabbit antibody to MPO
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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