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Code
OSR00220W
$240USD    Buy Now 

ID Tag
Rb2872-090916-WS

Immunogen
A synthetic peptide from human Rhodopsin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat.

Accession

Also known
Rhodopsin, opsin-2, RHO, OPN2, RP4

Target
FUNCTION: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
BIOPHYSICOCHEMICAL PROPERTIES:
Absorption: Abs(max)=495 nm;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE: Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP4 inheritance is autosomal dominant.
DISEASE: Defects in RHO are a cause of retinitis pigmentosa autosomal recessive (ARRP).
DISEASE: Defects in RHO are a cause of autosomal dominant congenital stationary night blindness 1 (CSNBAD1); also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a nonprogressive retinal disorder characterized by impaired night vision and ocular symptoms such as myopia, hyperopia, nystagmus and reduced visual acuity.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Nathans J, et al. Proc. Natl. Acad. Sci. U.S.A. 81:4851-4855(1984).

Limitation
For research use only

Related
products
 
Code Product Name
OSR00394G Rabbit antibody to Rhodopsin
 

Code
OSR00220W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1:2000 is recommended for IHC-P and 1:5000 to 1:7000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Rhodopsin.

Spcs X-react.
Human, mouse, rat, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSR00220W
$240USD    Buy Now 


Rabbit antibody to Rhodopsin IHC-P on paraffin sections of mouse eye. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Rhodopsin IHC-P on paraffin sections of rat eye. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Rhodopsin IHC-P on paraffin sections of rat eye. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Rhodopsin IHC-P on paraffin sections of rat eye. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Rhodopsin WB on mouse eye lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.
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Rabbit antibody to Rhodopsin
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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