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Code
OSS00005W
$260USD    Buy Now 

ID Tag
Sh13-281007-WS

Immunogen
Synthetic peptides from EGFP/GFP/Venus protein was used as the antigen.

Accession

Also known
Enhanced Green Fluorescence Protein

Target
GFP is a naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al.
GFP makes green light out of aequorin™s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen.
BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Tsien R. Y. Annu. Rev. Biochem. 67:509-544(1998).

Limitation
For research use only

Related
products

Code
OSS00005W
$260USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
Sheep

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Specificity
Specific for EGFP/GFP/Venus

Spcs X-react.
Any organism expressing EGFP/GFP/Venus.

Format
Lyophilised. This product contains 0.02% benzalkonium chloride.

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Sheep antibody to EGFP/GFP/Venus
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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