A synthetic peptide from human TAS2R5 conjugated to blue carrier protein was used as the antigen.
Taste receptor type 2 member 5, T2R5
This gene encodes a bitter taste receptor; bitter taste receptors are members of the G protein-coupled receptor superfamily and are specifically expressed by taste receptor cells of the tongue and palate epithelia. Each of these apparently intronless taste receptor genes encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes on chromosome 7 and is genetically linked to loci that influence bitter perception.
FUNCTION: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.
MISCELLANEOUS: Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Adler E, et al. Cell 100:693-702(2000).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 is recommended for WB and 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for TAS2R5.
Human, rat, marmoset, mouse. Other species not yet tested.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.