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Code
OSS00405W
$240USD    Buy Now 

ID Tag
Rb2332-290414-WS

Immunogen
A synthetic peptide from aa region 580-630 of human SLC6A8 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Sodium- and chloride-dependent creatine transporter 1, CT1, Creatine transporter 1, Solute carrier family 6 member 8, SLC6A8

Target
FUNCTION: Required for the uptake of creatine in muscles and brain.
Defects in SLC6A8 are the cause of X-linked creatine deficiency syndrome (XL-CDS). XL-CDS causes developmental delay, hypotonia, mental retardation, seizures, short stature and midface hypoplasia.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
Tissue specificity: Predominantly expressed in skeletal muscle and kidney. Also found in brain, heart, colon, testis and prostate.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Nash S.R, et al. Recept. Channels 2:165-174(1994)
2. Daub H, et al. Mol. Cell 31:438-448(2008)

Limitation
For research use only

Related
products

Code
OSS00405W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SLC6A8.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00405W
$240USD    Buy Now 


Rabbit antibody to SLC6A8 (580-630) IHC-P on paraffin sections of rat kidney.
The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SLC6A8 (580-630) IHC-P on paraffin sections of rat kidney. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
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Rabbit antibody to SLC6A8 (580-630)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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