⚬ Services
Code
OSH00052W
$240USD    Buy Now 

ID Tag
Rb2191-011013-WS

Immunogen
A synthetic peptide from aa region 40-84 of hydra Hydramacin 1 conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
Hm-1

Target
FUNCTION: Cationic antimicrobial peptide potently active against Gram-positive and Gram-negative bacteria including multi-resistant human pathogenic strains. Is not active against the Gram-positive Coccus species, Gram-negative non-fermentation species and against the fungus C.albicans. It leads to aggregation of bacteria as an initial step of its bactericidal mechanism. Aggregated cells are connected via electron-dense contacts and adopt a thorn apple-like morphology. Hydramycin contains a belt of positively charged residues that separate two hydrophobic areas. This structure may explain the observed aggregation of bacteria, since each of these areas can immerse into the outer leaflets of the membranes of two individual bacteria. Is able to permeabilize membranes of viable bacteria at low and neutral pH values, but no pore-forming activity is not detected.
SUBCELLULAR LOCATION: Secreted. Target cell membrane
Tissue specificity: Expressed in the endodermal epithelium.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Jung S, et al. J. Biol. Chem. 284:1896-1905(2009)

Limitation
For research use only

Code
OSH00052W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Hydramacin-1.

Spcs X-react.
Hydra. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to hydra Hydramacin 1 (40-84)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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