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Code
OSC00290W
$240USD
ID Tag
Rb2019-270213-WS
Immunogen
A synthetic peptide from aa region 100-150 of mouse CBX4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
Accession
Also known
E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2
Target
FUNCTION: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
SUBCELLULAR LOCATION: Nucleus. Nucleus speckle
Tissue specificity: Ubiquitous
SUBCELLULAR LOCATION: Nucleus. Nucleus speckle
Tissue specificity: Ubiquitous
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Kagey M.H, et al. EMBO J. 24:108-119(2005)
2. Vandamme J, et al. Mol. Cell. Proteomics 0:0-0(2011)
2. Vandamme J, et al. Mol. Cell. Proteomics 0:0-0(2011)
Limitation
For research use only
Code
OSC00290W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for CBX4.
Spcs X-react.
Human, mouse. Other species not yet tested but expected to work in rat.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSC00290W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Rabbit antibody to CBX4 (100-150)
