A synthetic peptide from aa region of 200-280 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, Eno1
Gamma enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, ENOG, NSE, Eno2
Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, MSE, Skeletal muscle enolase, Eno3
Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses
. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding
. In vitro, interacts with several glycolytic enzymes including PKM2, PGM, CKM and aldolase. Also binds troponin, in vitro.
Subcellular location: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band.
Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. In striated muscle, expression of ENO1 appears to be independent of fiber type.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. McAleese S.M, et al. Eur. J. Biochem. 178:413-417(1988)
For research use only
NZ white rabbit
Polyclonal, whole serum
WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for Enolase (alpha, beta, gamma).
Human, rat, mouse, marmoset. Other species not yet tested.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.