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Code
OSC00210W
$240USD
ID Tag
Rb1208-051209-WS
Immunogen
A synthetic peptide from the n-terminal of human Cullin 5 (Vasopressin activated calcium-mobilizing receptor 1) conjugated to blue carrier protein was used as the antigen.
Accession
Also known
CUL-5, Vasopressin-activated calcium-mobilizing receptor 1, VACM-1, VACM1
Target
Function: Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Byrd P.J, et al. Genome Res. 7:71-75(1997)
Limitation
For research use only
Code
OSC00210W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for Cullin5.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSC00210W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSC00210W
$240USD
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (Vasopressin activated calcium-mobilizing receptor 1): whole serum (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (Vasopressin activated calcium-mobilizing receptor 1): whole serum (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (Vasopressin activated calcium-mobilizing receptor 1): whole serum (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (Vasopressin activated calcium-mobilizing receptor 1): whole serum (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal of Cullin 5 (OSC00210W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. |
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