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Code
OSC00185W
$240USD    Buy Now 

ID Tag
Rb1188-260909-WS

Immunogen
A synthetic peptide from 2d cytoplasmic domain of mouse Connexin-57 (CX57, CXA10) conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
CXA10, Gap junction alpha-10 protein, Connexin-57, Connexin57, Connexin 57, CX57, GJA10, Connexin 62

Target
FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Involved in tracer coupling between horizontal cells of the retina. May play a role in the regulation of horizontal cell patterning.
Tissue specificity: Low levels were detected in skin, heart, kidney, testis, ovary, intestine. Expression not detected in brain, sciatic nerve or liver.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Manthey D, et al. J. Biol. Chem. 274:14716-14723(1999)
2. Church D.M, et al. PLoS Biol. 7:E1000112-E1000112(2009)
3. Hombach S, et al. Eur. J. Neurosci. 19:2633-2640(2004)
4. Soehl G, et al. Cell Commun. Adhes. 10:27-36(2003)
5. Ciolofan C, et al. Neuroscience 148:473-488(2007)

Limitation
For research use only

Code
OSC00185W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Connexin-57.

Spcs X-react.
Mouse. Other species not yet tested but expected to work in human.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to Connexin 57
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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