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Code
OSN00033G
$240USD    Buy Now 

ID Tag
Rb2327-290414-G

Immunogen
A synthetic peptide from extracellular domain of mouse NG2 conjugated to blue carrier protein was used as the antigen.

Accession

Also known
MEL CSPG, MELCSPG, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, CSPG4, MCSP, MCSPG, Melanoma associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MSK16

Target
Valuable marker for several incompletely differentiated precursor cells.
Function: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Subcellular location: Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection › lamellipodium membrane; Single-pass type I membrane protein; Extracellular side.
Tissue specificity: Expressed in microcascular pericytes and not endothelial cells.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Petersen C.M, et al. J. Biol. Chem. 272:3599-3605(1997).

Limitation
For research use only

Related
products
 
Code Product Name
OSN00019W Rabbit antibody to NG2
 

Code
OSN00033G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for NG2.

Spcs X-react.
Mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to NG2
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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