A synthetic peptide from the n-terminal region of human GARP conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
CNGB1, Cyclic nucleotide-gated cation channel beta-1, Cyclic nucleotide-gated channel beta-1, Cyclic nucleotide-gated cation channel 4, CNG channel 4, CNG-4, CNG4, Cyclic nucleotide-gated cation channel modulatory subunit, Glutamic acid-rich protein, GARP, Cyclic nucleotide-gated cation channel gamma, CNCG2, CNCG3L, CNCG4, RCNC2
FUNCTION: Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP. Isoform GARP2 is a high affinity rod photoreceptor phosphodiesterase (PDE6)-binding protein that modulates its catalytic properties: it is a regulator of spontaneous activation of rod PDE6, thereby serving to lower rod photoreceptor "dark noise" and allowing these sensory cells to operate at the single photon detection limit.
Subcellular location: Membrane; Multi-pass membrane protein
Involvement in disease: Defects in CNGB1 are the cause of retinitis pigmentosa type 25 (RP25). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Chen T.-Y, et al. Nature 362:764-767(1993)
2. Ardell M.D, et al. Genomics 28:32-38(1995)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for GARP.
Mouse, rat, human. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.