A synthetic peptide from the c-terminal region of human Fibrillarin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and xenopus.
FBRL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, FIB1, FLRN
FUNCTION: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.
Involvement in disease: Antisera from circa 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
SUBCELLULAR LOCATION: Nucleus âº nucleolus. Note: Fibrillar region of the nucleolus
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Jansen R.P, et al. J. Cell Biol. 113:715-729(1991)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for Fibrillarin.
Human, rat, mouse, xenopus. Not yet tested in other species.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.