A synthetic peptide from aa 140 -180 of human AQP0 conjugated to blue carrier protein was used as the antigen. The peptide shares 95% identity with rat, mouse and guinea pig sequences..
MIP, Lens fiber major intrinsic protein, Aquaporin-0, MIP26, MP26, MIP, AQP0
FUNCTION: Water channel. May be responsible for regulating the osmolarity of the lens.
Tissue specificity: Expressed exclusively in duodenum and jejunum. Highest expression in absorptive epithelial cells at the tips of villi in the jejunum. Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction âº gap junction
Tissue specificity: Major component of lens fiber gap junctions.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Pisano M.M, et al. Genomics 11:981-990(1991)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for AQP0.
Human. Other species not yet tested but expected to work in rat, mouse and Guinea pig.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.