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Code
OSA00101W
$240USD    Buy Now 

ID Tag
Rb1053-020809-WS

Immunogen
A synthetic peptide from aa 140 -180 of human AQP0 conjugated to blue carrier protein was used as the antigen. The peptide shares 95% identity with rat, mouse and guinea pig sequences..

Accession

Also known
MIP, Lens fiber major intrinsic protein, Aquaporin-0, MIP26, MP26, MIP, AQP0

Target
FUNCTION: Water channel. May be responsible for regulating the osmolarity of the lens.
Tissue specificity: Expressed exclusively in duodenum and jejunum. Highest expression in absorptive epithelial cells at the tips of villi in the jejunum. Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction
Tissue specificity: Major component of lens fiber gap junctions.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Pisano M.M, et al. Genomics 11:981-990(1991)

Limitation
For research use only

Code
OSA00101W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for AQP0.

Spcs X-react.
Human. Other species not yet tested but expected to work in rat, mouse and Guinea pig.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00101W
$240USD    Buy Now 


Rabbit antibody to AQP0 (140-180)

IHC on paraffin sections of human skin tissue using Rabbit antibody to AQP0 (140-180): OSA00101W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.


⚬ Products
Rabbit antibody to AQP0 (140-180)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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