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Code
OSA00095W
$240USD
ID Tag
Rb976-280609-WS
Immunogen
A synthetic peptide from the 3d extracellular domain of human AQP10 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
Aquaporin-10, AQP-10, AQP10, Aquaporin10, Small intestine aquaporin
Target
FUNCTION: Forms a water channel. Not permeable to urea and glycerol. May contribute to water transport in the upper portion of small intestine. Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Tissue specificity: Expressed exclusively in duodenum and jejunum. Highest expression in absorptive epithelial cells at the tips of villi in the jejunum.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Expressed exclusively in duodenum and jejunum. Highest expression in absorptive epithelial cells at the tips of villi in the jejunum.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ishibashi K, et al. Biochim. Biophys. Acta 1576:335-340(2002)
2. Gregory S.G, et al. Nature 441:315-321(2006)
2. Gregory S.G, et al. Nature 441:315-321(2006)
Limitation
For research use only
Code
OSA00095W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for AQP10.
Spcs X-react.
Human. Other species not yet tested.
Format
Lyophilised. This product contains 0.02% benzalkonium chloride.
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00095W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00095W
$240USD
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IHC-P on paraffin sections of human small intestine. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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