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Code
OSA00093W
$240USD
ID Tag
Rb974-280609-WS
Immunogen
A synthetic peptide from the n-terminal region of rat Aquaporin 8 (AQP8) conjugated to an immunogenic carrier protein was used as the antigen.
Accession
Also known
Aquaporin-8, AQP-8, AQP8, Aquaporin8
Target
FUNCTION: Forms a water-specific channel; mercury-sensitive. It may have an important role in spermatogenesis, in fertilization, and in the secretion of pancreatic juice and saliva.
Tissue specificity: Highly expressed in sperm, pancreas and liver. Some expression has been found in salivary gland and absorptive colonic epithelial cells. Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Highly expressed in sperm, pancreas and liver. Some expression has been found in salivary gland and absorptive colonic epithelial cells. Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ishibashi K, et al. Biochem. Biophys. Res. Commun. 237:714-718(1997)
2. Koyama Y, et al. J. Biol. Chem. 272:30329-30333(1997)
2. Koyama Y, et al. J. Biol. Chem. 272:30329-30333(1997)
Limitation
For research use only
Related
products
products
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Code
OSA00093W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for AQP8.
Spcs X-react.
Rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00093W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00093W
$240USD
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IHC on paraffin sections of rat testis tissue using Rabbit antibody to AQP8: OSA00093W. |
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IHC on paraffin sections of rat testis tissue using Rabbit antibody to AQP8: OSA00093W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat testis tissue using Rabbit antibody to AQP8: OSA00093W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat testis tissue using Rabbit antibody to AQP8: OSA00093W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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