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Code
OSC00089W
$240USD    Buy Now 

ID Tag
Rb809-140209-WS

Immunogen
A synthetic peptide from a cytoplasmic domain of human CACNA1I (Cav3.3) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Voltage-dependent T-type calcium channel subunit alpha-1I, Voltage-gated calcium channel subunit alpha Cav3.3

Target
Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Isoform alpha-1I gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Gates in voltage ranges similar to, but higher than alpha 1G or alpha 1H.
Subcellular location: Membrane; Multi-pass membrane protein.
Tissue specificity: Brain specific

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Mittman S, et al. Neurosci. Lett. 269:121-124(1999)
2. Monteil A, et al J. Biol. Chem. 275:16530-16535(2000)

Limitation
For research use only

Related
products
 
Code Product Name
OSC00115G Rabbit antibody to CACNA1I
 

Code
OSC00089W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specificity
Specific for CACNA1I.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSC00089W
$240USD    Buy Now 


Rabbit antibody to CACNA1I IHC on rat brain (paraffin section) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 500 dilution incubated overnight at 4C. Nerve fibers are intensely stained.
Rabbit antibody to CACNA1I IHC on rat brain (paraffin section) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 500 dilution incubated overnight at 4C.
Rabbit antibody to CACNA1I IHC on rat brain (paraffin section) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 500 dilution incubated overnight at 4C.
Rabbit antibody to CACNA1I IHC on rat brain (paraffin section) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 500 dilution incubated overnight at 4C.
Rabbit antibody to CACNA1I IHC on rat brain (paraffin section) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 500 dilution incubated overnight at 4C.
Rabbit antibody to CACNA1I IHC on rat brain (paraffin section) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 500 dilution incubated overnight at 4C.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
Rabbit antibody to CACNA1I IHC on rat spinal cord (free floating sections) using Rabbit antibody to CACNA1I (OSC00089W) at 1 : 300 dilution incubated overnight at 4C and developed with DAB/Ni.
⚬ Products
Rabbit antibody to CACNA1I
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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